Coding

Part:BBa_K1991004:Design

Designed by: Chen, Pei-En   Group: iGEM16_Mingdao   (2016-10-10)


Lpp-OmpA-BamHI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 436
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 391
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

BBa_K1991004: Lpp-OmpA-BamHI/pSB1C3 The DNA fragment of Lpp-OmpA was amplified by PCR using BBa_K1694035 as a template with a primer containing BamHI site and digested by EcoRI and SpeI, followed by cloning onto pSB1C3 which was cut by EcoRI and SpeI. The part has been confirmed by sequencing.



Source

Modified based on BBa_K1694002 (Lpp-OmpA-N)

References